Addgene

We narrowed to 1,564 results for: GC1

Showing: 741 - 760 of 1564 results

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pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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HOXA4 (murine) pET

Plasmid

#8488

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
TAL3583

Plasmid

#47302

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-trappc11-right (foigr Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
TAL3582

Plasmid

#47301

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-trappc11-left (foigr Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
GFP-hPLEKHA6-Myc

Plasmid

#236540

Purpose

For expression of PLEKHA6 (human) in mammalian cells

Depositor

Sandra Citi

Article

Sluysmans et al Mol Biol Cell. 2021 Nov 1;32(21):ar34. d

Insert

PLEKHA6 (PLEKHA6 Human)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN

Plasmid

#236511

Purpose

For expression of GFP-Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN (Cgn Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

Plasmid

#233546

Purpose

A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

Plasmid

#232132

Purpose

A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

FOXF1 (FOXF1 Human)

Use

Gateway cloning

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-dgat2 in situ probe

Plasmid

#232134

Purpose

zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat2 (dgat2 Zebrafish)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

Plasmid

#217716

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-862

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

Plasmid

#217683

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-856

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK5_IRS

Plasmid

#109797

Purpose

Protein expression and purification of DOK5_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK5_IRS (DOK5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_C_COX14

Plasmid

#111663

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

COX14 (C12orf62 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP2-2/3

Plasmid

#91468

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-2/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP2-3/3

Plasmid

#91411

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-3/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP2-1/3

Plasmid

#91388

Purpose

Protein expression and purification of human SH3 domain construct RIMBP2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP2-1/3 (RIMBP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1E-1/1-SV1/2

Plasmid

#91324

Purpose

Protein expression and purification of human SH3 domain construct MYO1E-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1E-1/1-SV1/2 (MYO1E Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1E-1/1-SV2/2

Plasmid

#91259

Purpose

Protein expression and purification of human SH3 domain construct MYO1E-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1E-1/1-SV2/2 (MYO1E Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA8

Plasmid

#19514

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 8 (HSPA8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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TFORF1998

Plasmid

#142307

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF248 (ZNF248 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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