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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 772 results for: NEB

Showing: 741 - 760 of 772 results

  1. pChlr-4NYQ

    Plasmid
    #52157
    Purpose
    Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 13H to 13Y in Module 4
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pChlr-5SE

    Plasmid
    #52160
    Purpose
    Encodes module 5 with amino acids 12S-16E for recognition of nt 4G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 5
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pChlr-5NQ

    Plasmid
    #52161
    Purpose
    Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 5
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pChlr-6CQ

    Plasmid
    #52163
    Purpose
    Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 6
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pChlr-6SR

    Plasmid
    #52166
    Purpose
    Encodes module 6 with amino acids 12S-16R for recognition of nt 3C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 6
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pChlr-4NQ

    Plasmid
    #52153
    Purpose
    Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52153-plasmid-map-sequence-id-80994

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  7. pChlr-5CQ

    Plasmid
    #52159
    Purpose
    Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52159-plasmid-map-sequence-id-80995

    Enlarge

  8. pChlr-6NQ

    Plasmid
    #52165
    Purpose
    Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52165-plasmid-map-sequence-id-80996

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  9. pChlr-8NQ

    Plasmid
    #52173
    Purpose
    Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    52173-plasmid-map-sequence-id-80998

    Enlarge

  12. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Expression
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

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  13. pUBC_HA_PP7-mCherry

    Plasmid
    #107605
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mCherry
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    hUBC
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    107605-plasmid-map-sequence-id-207484

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  14. pU6_T7_INT[6xminimal_Puf1]

    Plasmid
    #107608
    Purpose
    Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    6x minimal Puf1 motif
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107608-plasmid-map-sequence-id-207490

    Enlarge

  15. pCarCBA

    Plasmid
    #117754
    Purpose
    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117754-plasmid-map-sequence-id-229023

    Enlarge

  16. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

    Enlarge

  19. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

    Enlarge

  20. pCarCBAE

    Plasmid
    #117755
    Purpose
    Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac(UV5)
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    117755-plasmid-map-sequence-id-229025

    Enlarge

Showing: 741 - 760 of 772 results