We narrowed to 8,028 results for: lenti crispr plasmid

1. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5d.SFFV.Cas9.P2A.BSD

   Plasmid

   #181981

   Purpose

   Lentiviral Cas9 expression

   Depositor

   Jan-Henning Klusmann

   Insert

   Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FU_tetO_dCas9-VP192-T2A-EGFP

   Plasmid

   #121173

   Purpose

   Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

   Depositor

   Timo Otonkoski

   Insert

   dCas9-VP192-T2A-EGFP
   

   Use

   CRISPR and Lentiviral; Inducible by doxycycline

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM808

   Plasmid

   #134181

   Purpose

   Lentiviral sgRNA plasmid with blasticidin resistance

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR Ef1a-dCas9-BFP-KRAB

   Plasmid

   #217304

   Purpose

   lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

   Depositor

   Luke Gilbert

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX_TRC311

   Plasmid

   #113668

   Purpose

   Gateway destination vector for lentiviral expression

   Depositor

   John Doench

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CROPseq-EFS-SpCas9-P2A-blast

   Plasmid

   #106477

   Purpose

   single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

   Depositor

   Alex Hewitt

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

   Plasmid

   #102245

   Purpose

   Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

   Depositor

   Jacob Corn

   Insert

   dCas9-VPR-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-VPR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX_TRC311

    Plasmid

    #113668

    Purpose

    Gateway destination vector for lentiviral expression

    Depositor

    John Doench

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid

    #102245

    Purpose

    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

    Depositor

    Jacob Corn

    Insert

    dCas9-VPR-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-VPR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid

    #221385

    Purpose

    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

    Depositor

    Junwei Shi

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid

    #102244

    Purpose

    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

    Depositor

    Jacob Corn

    Insert

    dCas9-HA-BFP-KRAB-NLS
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-TagBFP-KRAB-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA.SFFV.tBFP

    Plasmid

    #169940

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA.SFFV.tRFP

    Plasmid

    #169941

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA.SFFV.GFP

    Plasmid

    #169938

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sgRNA.SFFV.RFP657

    Plasmid

    #169939

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and RFP657 from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge