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pSCrhaB2_ApyD_ RBS_ApyAOHIS

Plasmid

#226247

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A1.1-GFP (pc2188)

Plasmid

#223707

Purpose

Expression of macroH2A1.1 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.1-GFP (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo25

Plasmid

#203995

Purpose

Cloning plasmid containing the Hro ES6 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES6

Expression

Plant

Promoter

Hro ES6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo23

Plasmid

#203993

Purpose

Cloning plasmid containing the Hro ES4 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES4

Expression

Plant

Promoter

Hro ES4

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-EGFP

Plasmid

#210318

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM106B-2Strep

Plasmid

#210360

Purpose

Expresses TMEM106B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-4gRNA-GBX2-RFP

Plasmid

#192288

Purpose

Expresses RFP and four sgRNAs against GBX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

RFP and four sgRNA GBX2 (GBX2 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo14

Plasmid

#203984

Purpose

Cloning plasmid containing the Elu ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES1

Expression

Plant

Promoter

Elu ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo16

Plasmid

#203986

Purpose

Cloning plasmid containing the Elu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES3

Expression

Plant

Promoter

Elu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo18

Plasmid

#203988

Purpose

Cloning plasmid containing the Ppu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES2

Expression

Plant

Promoter

Ppu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo19

Plasmid

#203989

Purpose

Cloning plasmid containing the Ppu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES3

Expression

Plant

Promoter

Ppu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo22

Plasmid

#203992

Purpose

Cloning plasmid containing the Hro ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES3

Expression

Plant

Promoter

Hro ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2

Plasmid

#204576

Purpose

Expresses 3xFLAG-p62 var in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

None

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mut GBP2 V5-Twist

Plasmid

#201377

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y107E

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-ELuc-9

Plasmid

#197736

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Emerald/Enhanced Click Beetle Luciferase (ELuc) (tomato codon-optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsNot7_T

Plasmid

#147637

Purpose

Bacterial Expression of HsNot7

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot7 (CNOT7 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Tags

6His

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

DHFR P66L

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-T11HA-hMOV10-WT

Plasmid

#178907

Purpose

expression of WT hMOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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