We narrowed to 17,085 results for: Por

1. pMXS-IRES-Blast CHKA

   Plasmid

   #218540

   Purpose

   human CHKA cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CHKA (CHKA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

   Plasmid

   #90009

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TEK-V5/HIS

   Plasmid

   #205611

   Purpose

   Expression of human TEK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TEK receptor tyrosine kinase (TEK Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PM-RspA-CFAST

   Plasmid

   #233601

   Purpose

   Expression of RspA-CFAST on the PM facing the cytosol

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-CFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.GFP-KIF16B tail 458-1317

   Plasmid

   #134624

   Purpose

   Mammalian expression of human KIF16B 458-1317

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF16B (KIF16B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 457 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ppk301-T2A-QF2 HDR plasmid

   Plasmid

   #130667

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK 3xFLAG-KPNB1 zeo

   Plasmid

   #192326

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-KPNB1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNB1 (KPNB1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD8-mCherry-FRB

   Plasmid

   #100738

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   FRB and mCherry

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194686

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Use

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215326

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    dNTP63 Promoter
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dNTP63

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Use

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

    Plasmid

    #138417

    Purpose

    CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Inserts

    BirA
    dCas9
    

    Use

    Lentiviral

    Tags

    FLAG, Avi-tag and V5, His

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IP/SECFP-hATG16A1

    Plasmid

    #58994

    Purpose

    Expresses SECFP-hATG16A1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Expression

    Mutation

    codon optimized for human

    Promoter

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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