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GFP-PODXL S481A

Plasmid

#195223

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

S481A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481D

Plasmid

#195224

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

S481D

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGI_Myr-YFP-MiniTurboID

Plasmid

#222432

Purpose

Potential plasma membrane control.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker, Myr, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHMTc-FBF-2 (121-632)

Plasmid

#218664

Purpose

Expression of MBP:6xHIS:FBF-2(121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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Src(KD/YF)-SsrA(Y7I)-mCer3

Plasmid

#221000

Purpose

this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

D388R/Y529F in Src, Y7I in SsrA

Promoter

pCMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJK2097

Plasmid

#218665

Purpose

Expression of MBP:6xHIS:FBF-2(Y479A, 121-632)

Depositor

Judith Kimble

Article

Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

Insert

fbf-2 (fbf-2 Nematode)

Tags

MBP-6xHIS

Expression

Bacterial

Mutation

amino acids 121-632 only, with Y479A mutation

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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MCAM-BirA*-HA

Plasmid

#214472

Purpose

Lentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

MCAM (MCAM Human)

Use

Lentiviral; Gateway

Tags

BirA(R118G) and HA tag

Expression

Mammalian

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNKX2-5-GFP-T2A

Plasmid

#203608

Purpose

Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC28-HiBIT-WDR5

Plasmid

#200883

Purpose

Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-WDR5 (WDR5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 1

Plasmid

#193608

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 1 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 1

Plasmid

#193600

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 1 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 2

Plasmid

#193601

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 2 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 1

Plasmid

#193589

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 1 (FADD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 2

Plasmid

#193590

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 2 (FADD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304 PRDM14

Plasmid

#193688

Purpose

Constitutive lentiviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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