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pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-dCas13b-ADAR2DD

Plasmid

#190913

Purpose

Addgene #103871 with 3x FLAG on N-terminal of dCas13

Depositor

Timothy Jarome

Article

Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

Insert

3X FLAG

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

Plasmid

#175801

Purpose

Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

AviTag, Hexahistidine tag, and eGFP

Expression

Bacterial

Mutation

E184Q W330Y

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00028 (aka pTS0057)

Plasmid

#200832

Purpose

AAVS1 guide RNA cloned into the lentiSAM puro backbone

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

AAVS1 guide RNA

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

Plasmid

#139446

Purpose

Lentiviral vector genome to ectopically express Firefly luciferase

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag Firefly luciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98928

Purpose

Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1 and AAV5

Insert

Ruby2-smFP-Flag

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB506

Plasmid

#74848

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp1-mut

Plasmid

#188568

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD

Plasmid

#70129

Purpose

Express flag-tagged mouse Tet1 catalytic domain.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain.

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP WT

Plasmid

#123097

Purpose

Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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H-IRF7

Plasmid

#169785

Purpose

Express IRF7 in bacteria

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

interferon regulatory factor 7 (IRF7), transcript variant a (IRF7 Human)

Tags

His tag

Expression

Bacterial

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB502-omega

Plasmid

#74845

Purpose

Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

2xCaMV35S enhancer and omega

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-mCherry

Plasmid

#220353

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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HC078 (3xHA-Atg13)

Plasmid

#59544

Purpose

Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

Depositor

Daniel Klionsky

Article

Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

Insert

ATG13 (ATG13 Budding Yeast)

Tags

3xHA

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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