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CtkA_D155Q/D179Q_pET21a

Plasmid

#27432

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Mutation

D155Q/D179Q

Available Since

May 2, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Flag-Tipin-L195A

Plasmid

#23120

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag- and His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin-L195A

Plasmid

#23121

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pTarget_dnaA-mEos4b

Plasmid

#212607

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein mEos4b, together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

mEos4b

Use

CRISPR

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pTarget_dnaA-PAmCherry2.1

Plasmid

#212605

Purpose

Contains the E. coli codon-optimised gene of the photoactivatable fluorescent protein PAmCherry2(M10L), together with a sgRNA for SpydCas9 targeting the E. coli dnaA gene.

Depositor

John van der Oost

Article

Olivi et al Nat Commun. 2025 Aug 21;16(1):7813. doi:

Insert

PAmCherry2.1

Use

CRISPR

Mutation

M10L

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_FV-NUP62-EGFP3

Plasmid

#252747

Purpose

Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore in absence of Rapalog2.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

3xEGFP and FV

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_Rev-GR(m10)-EGFP

Plasmid

#252751

Purpose

Control for studying nuclear export. Expression of a fusion protein containing full-length Rev, the hormone-responsive element of the rat Gr, and GFP. The m10 mutation abolishes nuclear export.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

HIV-1 Rev and Glucocorticoid Receptor (GR)

Tags

EGFP

Expression

Mammalian

Mutation

m10 is mutation of nuclear export sequence

Available Since

March 19, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pET01-NRF1-Ex7-minigene

Plasmid

#237459

Purpose

Vector for constitutive expression of NRF1 alternative exon-7 minigene

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7 minigene

Expression

Mammalian

Promoter

RSV LTR

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLCHKOv4

Plasmid

#237451

Purpose

Lentiviral backbone for expressing U6-driven hybrid guide (hg)RNAs for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLCHKOv5

Plasmid

#237452

Purpose

Lentiviral backbone for expressing doxycycline (Dox)-inducible, Pol II-driven hybrid guide (hg)RNAs.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli DNA Polymerase III subunit Delta' (holB) in pet28PP

Plasmid

#217410

Purpose

Expresses E. coli DNA Polymerase III subunit delta' (holb) with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

DNA polymerase III subunit Delta' (holB E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBK1579

Plasmid

#239794

Purpose

pLV-U6-[empty]-EFSNC-dSaCas9-DNMT3a-P2A-Puro-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[empty]-EFSNC-dSaCas9-DNMT3a-P2A-Puro-WPRE

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1576

Plasmid

#239796

Purpose

pLV-U6-[empty]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE (no gRNA vector)

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[empty]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1841

Plasmid

#239797

Purpose

pLV-U6-[SNCAintron1 gRNA 1]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE (THERAPEUTIC VECTOR)

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[SNCAintron1 gRNA 1]-EFSNC-dSaCas9-KRAB-MeCp2-P2A-Puro-WPRE

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK546

Plasmid

#239839

Purpose

pLV-U6-[hSNCA gRNA 4]-EFSNC-dSpCas9-DNMT3a-P2A-PURO-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[hSNCA gRNA 4]-EFSNC-dSpCas9-DNMT3a-P2A-PURO-WPRE

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2

Plasmid

#242812

Purpose

Lentiviral vector expressing mouse Pkm2 tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NES-CAGEc-AF9c (Npu)

Plasmid

#241413

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NES-CAGEc-AF9c (Npu) (MLLT3 Human, Synthetic)

Tags

3xFLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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