We narrowed to 14,951 results for: pET

1. pRK5-HA-SZT2

   Plasmid

   #87035

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rogi2-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179865

   Purpose

   Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pegfp-N1-TFEB-S3A,R4A

   Plasmid

   #44446

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed both Serine 3 and Arginine 4 to Alanine

   Promoter

   CMV

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SSB-wt

   Plasmid

   #197260

   Purpose

   wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

   Depositor

   Timothy Lohman

   Article

   Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

   Insert

   wt E. coli SSB
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag pLJM1 Rap2A

   Plasmid

   #19311

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RAP2A, member of RAS oncogene family (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5 Flag PRAS40

   Plasmid

   #14950

   Depositor

   David Sabatini

   Article

   Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

   Insert

   PRAS40 (AKT1S1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pRK5-HA GST RagC 120L

   Plasmid

   #19306

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding C (RRAGC Human)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Q120L

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGal4AD-CIBN

   Plasmid

   #28246

   Purpose

   Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1 (1-171aa)
   

   Use

   Tags

   Gal4AD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAF

   Plasmid

   #104152

   Purpose

   Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

   Depositor

   Robert Craigie

   Article

   Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

   Insert

   Barrier-to-autointegration factor (BAF) (BANF1 Human)
   

   Use

   Tags

   N-terminal His tag with thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hsyn-FLPo-T2A-GFP

    Plasmid

    #161766

    Purpose

    express FLPo and GFP under the control of human synapsin promoter

    Depositor

    Baoji Xu

    Article

    An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

    Inserts

    FLPo
    T2AEGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. KBB418

    Plasmid

    #185088

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 RagC shRNA #1

    Plasmid

    #26629

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-HA GST RagA 66L

    Plasmid

    #19300

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding A (RRAGA Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Q66L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-Flag-CtIP

    Plasmid

    #71108

    Purpose

    mammalian expession of Flag-CtlP

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pLKO.1 RagC shRNA #2

    Plasmid

    #26630

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pHyg-yeGFP-His6

    Plasmid

    #212781

    Purpose

    C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-Cre

    Plasmid

    #125574

    Purpose

    Expresses Cre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-HA GST RagA 21L

    Plasmid

    #19299

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding A (RRAGA Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    T21L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only