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CD8a-AA347-363-KRN-3A-GFP

Plasmid

#221598

Purpose

Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with KRN-3A mutation fused to the cytosolic tail and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 347-363 are inserted with mutation of…

Promoter

CMV promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTW368z

Plasmid

#246581

Purpose

Golden gate acceptor for AtRaf1 library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

sfGFP, AtRaf2, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (RbcX2, CPN60B, CPN20, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e3

Plasmid

#246588

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Nov. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 GST-SHP2 H116A E252A

Plasmid

#244493

Purpose

Bacterial Expression of pH insensitive SHP2 mutant

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

PTPN11 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Histidine 116 to Alanine and Glutamate 25…

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411a

Plasmid

#246582

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN20, CPN60A, AT3G04550 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

Plasmid

#233046

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry

Tags

mCherry

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

Plasmid

#233045

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry

Tags

mCherry

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRP.USH2A

Plasmid

#226555

Purpose

Minigene encoding USH2A exon 13 and surrounding introns/exons

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

USH2A (USH2A Human)

Expression

Mammalian

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA2

Plasmid

#223522

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA1

Plasmid

#223521

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4-del974-1265_V

Plasmid

#147786

Purpose

Mammalian Expression of HsEDC4-del974-1265

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsEDC4-del974-1265 (EDC4 Human)

Expression

Mammalian

Mutation

one non silent mutation I286T compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG11-COMP5AP-AviTag-9xHis

Plasmid

#157304

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG11 (PSG11 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PSG9-COMP5AP-AviTag-9xHis

Plasmid

#157388

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PSG9 (PSG9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-IST1 N-terminal domain

Plasmid

#184805

Purpose

Bacterial expression of IST1 N-terminal domain

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

IST1 (IST1 Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

aa 1-187 only

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His-MBP-CHMP1B, S2C

Plasmid

#184803

Purpose

Bacterial expression of CHMP1B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

CHMP1B (CHMP1B Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSUMO GLTP

Plasmid

#170732

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

GLTP (GLTP Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA targeting human SLC12A5 gene stop codon

Plasmid

#158577

Purpose

For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

Depositor

Rudolf Jaenisch

Article

Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

Insert

sgRNA targeting human SLC12A5 gene stop codon

Use

CRISPR

Tags

Cas9 and GFP

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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