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pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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dFP

Plasmid

#120542

Purpose

Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein (dFP)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-FLP(D)

Plasmid

#119895

Purpose

FLP(D) with a hsp70 promoter

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Insert

FLP(D)

Use

Drosophila expression

Mutation

mutated form of FLP, which at position 5 contains…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-VP64-2AmScarlet

Plasmid

#121963

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64 module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Tags

HA

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE1-VP64-P10

Plasmid

#104606

Purpose

Expresses TALE1 under UAS control

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

TALE1

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Kaptin

Plasmid

#100511

Purpose

Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

KPTN (KPTN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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CDk4-luciferase fragment A

Plasmid

#86658

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

generated by cleavage at the NcoI site from 880 (…

Promoter

CDK6

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-irp9 WT

Plasmid

#87105

Purpose

expresses Irp9 WT, the salicylate synthase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Nov 1;539(1):

Insert

irp9 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-C1-GEFT

Plasmid

#84340

Purpose

Expresses p63 (474 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

GEFT (ARHGEF25 Human, Synthetic)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH-SWAP70(R223E, R224E)

Plasmid

#84583

Purpose

Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306, mut…

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-rNanog-pgk-hph

Plasmid

#74917

Purpose

Mammalian expression of rNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Rat)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK240

Plasmid

#71127

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL21

Plasmid

#51795

Purpose

Inducible expression of TAL21 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL21

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-ABM-10

Plasmid

#57421

Purpose

Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin AMB

Tags

mEos2

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUASTattb AaegOr4-E

Plasmid

#59796

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele E

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele E

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-C

Plasmid

#59794

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele C

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele C

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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