We narrowed to 4,342 results for: puc19

1. puC19 preACT1(del6) AcAc

   Plasmid

   #111332

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   3' splice site AcAc

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pre-Ubc4 BrG pUC19

   Plasmid

   #111322

   Purpose

   for transcription to Ubc4 pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

   Insert

   UBC4 (UBC4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   intron has branch-site A to G mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19 gene 60 thru gap

   Plasmid

   #49160

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Use

   Tags

   Expression

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   branch site A259C

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. puC19 preACT1(del6) C256A

   Plasmid

   #111331

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   branch site C256A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19 - T7 pro - IRES - EGFP

   Plasmid

   #138586

   Purpose

   Express EGFP with an upstream T7 promoter in pUC19 backbone

   Depositor

   Fei Chen

   Article

   Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_msfGFP-EWSR1-repair-tempate

   Plasmid

   #237682

   Purpose

   For knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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