We narrowed to 13,276 results for: SHI

1. pGEN25-Ub Hs

   Plasmid

   #83232

   Purpose

   pHis-par2-Ub Hs

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Ubiqutin (UBC Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Myc-PGK-Puro-IRES-GFP

   Plasmid

   #75124

   Purpose

   Retroviral expression plasmid encoding wild type murine Myc

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. lentiCRISPR - EGFP sgRNA 6

   Plasmid

   #51765

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 6
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSyn1-AsCpf1(RR)

   Plasmid

   #109325

   Purpose

   AAV vector expressing AsCpf1 (RR variant)

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   hAsCpf1(RR)
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mutation

   S542R/K607R

   Promoter

   hSyn1

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

   Plasmid

   #194750

   Purpose

   To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

   Plasmid

   #75118

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Bromodomain replaced with the bromodomain of BRD7

   Promoter

   Available since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSF11-wmiRFP2-T2A-HO1

   Plasmid

   #179485

   Purpose

   Expresses miRFP2 and HO1in worm cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   wmIRFP2-T2A-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   tag-168 promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

   Plasmid

   #191484

   Purpose

   Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-mScarlet-SARS-CoV-2-SL1 alone

    Plasmid

    #191485

    Purpose

    Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-AID'-seBE4max-IRES

    Plasmid

    #174697

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    AID'-seBE4max-IRES (AICDA Human)
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    UGI-E11K

    Promoter

    CMV

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-miRFP2

    Plasmid

    #178968

    Purpose

    Expresses miRFP2 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    AAV

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CAG

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75120

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the first bromodomain o…

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

16. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

    Plasmid

    #75116

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    single amino acid mutation N216A

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

17. MSCV-NSD3-short W284A

    Plasmid

    #72551

    Purpose

    expresses 3*FLAG tagged human NSD3-short with W284A mutation

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    W284A

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176460

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-crRNA(Non-targeting)

    Plasmid

    #109324

    Purpose

    AAV vector expressing non-targeting AsCpf1 crRNA

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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