We narrowed to 24,827 results for: Spr

1. msHook2 g2 lentiCRISPRv2-mCherry

   Plasmid

   #218653

   Purpose

   Knockout vector for mouse Hook2

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Hook2 (Hook2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GOLIM4 g1 LentiCRISPRv2-mCherry

   Plasmid

   #218662

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR-cBEST-v2-SF14P

   Plasmid

   #209448

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; sgRNA expression from SF14P promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available Since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2-bleo-COX1-4

   Plasmid

   #184722

   Purpose

   COX1-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog COX1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPRv2-hyg2-COX2-4

   Plasmid

   #184723

   Purpose

   COX2-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog COX2 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2-bleo-PTGER2

   Plasmid

   #184724

   Purpose

   EP2-KO in MDCK

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   A gRNA targeting the dog EP2 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2-bleo-PLA2G4A_3

    Plasmid

    #184725

    Purpose

    cPLA2-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog cPLA2 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2-hyg2-PTGER4

    Plasmid

    #184726

    Purpose

    EP4-KO in MDCK

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    A gRNA targeting the dog EP4 gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPR-v2 hygro sgUCK1

    Plasmid

    #211524

    Purpose

    Deletes UCK1

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    sgUCK1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAf-CRISPR-ctfR1

    Plasmid

    #207992

    Purpose

    CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    ctfR1
    

    Use

    CRISPR

    Promoter

    Aspergillus flavus U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv2-bleo-ErbB4

    Plasmid

    #197353

    Purpose

    A knock-out vector for dog ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRa_all-in-one_Bip gRNA1

    Plasmid

    #183696

    Purpose

    Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Insert

    gRNA targeting chinese hamster Bip (Hspa5)
    

    Use

    CRISPR

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-HISMX6-pSPR3

    Plasmid

    #191792

    Purpose

    Yeast gene induction with meiotic promoter

    Depositor

    Dean Dawson

    Insert

    HIS3 pSPR3
    

    Use

    Yeast genomic targeting

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-kanMX6-pSPR3

    Plasmid

    #191788

    Purpose

    Yeast gene induction with meiotic promoter

    Depositor

    Dean Dawson

    Insert

    KanMX pSPR3
    

    Use

    Yeast genomic targeting

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR2_+10D+10U

    Plasmid

    #149394

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    10bp inserted upstream and downstream of the IHFp…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ-NF1-L1-dSpRY

    Plasmid

    #177681

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ-NF1-L2-dSpRY

    Plasmid

    #177682

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dCas9-SpRY
    

    Use

    CRISPR; Gateway compatible foki-dcas9-spry entry …

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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