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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,589 results for: eng

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Showing: 7781 - 7800 of 14589 results

  6. pMP315

    Plasmid
    #134994
    Purpose
    KRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-KRAB-NLS-DpnI(aa146-254)
    Tags
    DpnI(aa146-254) and KRAB domain
    Expression
    Mammalian
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134994-plasmid-map-sequence-id-269997

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  12. pGG184

    Plasmid
    #165604
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    'CAGCG' PAM replacing the 'CGGCG&#…
    Promoter
    lac
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165604-plasmid-map-sequence-id-334628

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  13. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  14. IBMc071

    Plasmid
    #161943
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161943-plasmid-map-sequence-id-327544

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  15. pJAID5XY

    Plasmid
    #165051
    Purpose
    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Publication
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165051-plasmid-map-sequence-id-327149

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  16. pJAID5VY

    Plasmid
    #165048
    Purpose
    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Publication
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165048-plasmid-map-sequence-id-327148

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  19. pLY134

    Plasmid
    #130962
    Purpose
    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB5
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130962-plasmid-map-sequence-id-257714

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  20. HA-IFITM3-V93-TAG

    Plasmid
    #122479
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122479-plasmid-map-sequence-id-237724

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Showing: 7781 - 7800 of 14589 results