PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||165604||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)XL1 Blue
Copy numberLow Copy
Gene/Insert namehEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
Mutation'CAGCG' PAM replacing the 'CGGCG' PAM in pGHUC_R25
- Promoter lac
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ATGAGTAGCAGCACGTTCCTTA
- 3′ sequencing primer CCAGAGCATGTATCATATGGTCCAGAAACCC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGG184 was a gift from Marcus Noyes (Addgene plasmid # 165604 ; http://n2t.net/addgene:165604 ; RRID:Addgene_165604)
For your References section:Engineered dual selection for directed evolution of SpCas9 PAM specificity. Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa DM, Boeke JD, Noyes MB. Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x. 10.1038/s41467-020-20650-x PubMed 33441553