pGG184
(Plasmid #165604)

• Purpose: Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP
• Depositing Lab: Marcus Noyes
• Publication: Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x.

Backbone

• Vector backbone: pGHUC_R25
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
• Mutation: 'CAGCG' PAM replacing the 'CGGCG' PAM in pGHUC_R25
• Promoter: lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ATGAGTAGCAGCACGTTCCTTA
• 3′ sequencing primer: CCAGAGCATGTATCATATGGTCCAGAAACCC

Resource Information

• Supplemental Documents:
  • pGG184.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry