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pGEX Vrap-SH2

Plasmid

#46531

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vrap (SH2D2A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 93-192

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Tyk2-SH2

Plasmid

#46527

Depositor

Bruce Mayer

Publication

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tyk2 (TYK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 452-567

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDASH-DI-ω1

Plasmid

#242517

Purpose

Donor I vector in DASH and omega1 vector in GoldenBraid/DASH, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-α1

Plasmid

#242515

Purpose

Donor II vector in DASH and alpha1 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Publication

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC0_N-mEGFP

Plasmid

#231255

Purpose

For expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_N-mEGFP

Plasmid

#231271

Purpose

For stably expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA MPG

Plasmid

#237804

Purpose

Lentiviral vector expressing WT MPG

Depositor

David Pellman

Publication

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

MPG (MPG Human)

Tags

Flag 3HA

Expression

Mammalian

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-PEX26

Plasmid

#224557

Purpose

expresses Keima fused PEX26 peroxisomal localization sequence in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Publication

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

Keima-PEX26 (PEX26 Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXS-IP APEX-PEX26

Plasmid

#224562

Purpose

expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Publication

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX26 (PEX26 Human)

Use

Retroviral

Tags

APEX

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

Plasmid

#223716

Purpose

Expresses FLAG-TurboID-dPB1-SQSTM1

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 1-119

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

Plasmid

#223717

Purpose

Expresses FLAG-TurboID-dUBA-SQSTM1

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 384-Stop codon

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_N_CCDC93

Plasmid

#209687

Purpose

Expresses MAC-tagged CCDC93 in mammalian cells

Depositor

Markku Varjosalo

Publication

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

CCDC93 (CCDC93 Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_VPS26C

Plasmid

#209689

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Publication

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-chmI

Plasmid

#221341

Purpose

Express ChmI in E. coli

Depositor

Emily Balskus

Publication

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmI

Tags

His6

Expression

Bacterial

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Publication

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHelper_AraC/pBad-Tnpase (camR)

Plasmid

#213909

Purpose

Arabinose-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Publication

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

AraC/pBad ATc-inducible himar1C9 transposase

Expression

Bacterial

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PB-NLS-ZapCV2

Plasmid

#203664

Purpose

Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

Depositor

Amy Palmer

Publication

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2 Zinc sensor

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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PB-NLS-ZapCV2-ALA

Plasmid

#203665

Purpose

Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

Depositor

Amy Palmer

Publication

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRO16Sh2

Plasmid

#180604

Purpose

Expression of ShCAST under control of Plac with pRO1600 origin.

Depositor

Christopher Reisch

Publication

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLac

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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