We narrowed to 59,446 results for: cloning

1. pLenti_rtTA-P2A-BFP_HygroR

   Plasmid

   #186738

   Purpose

   rtTA-P2A-BFP expression construct cloned into lentiviral backbone

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   rtTA
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19/N-Vc

   Plasmid

   #183157

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO V5 HSPH1

   Plasmid

   #19506

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 105kDa/110kDa protein 1 (HSPH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT.000

   Plasmid

   #119559

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

   Plasmid

   #114322

   Purpose

   Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO V5 HSPA1A

   Plasmid

   #19510

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 1A (HSPA1A Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19/Vc-C

   Plasmid

   #183158

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vc.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. epi-ABEmax-NG

   Plasmid

   #135976

   Purpose

   Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

   Depositor

   Yongming Wang

   Article

   Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pLBPVXBa-M

   Plasmid

   #229079

   Purpose

   New version of a PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Expression

   Plant

   Mutation

   Mutation coat protein start codon (ATG to AGG); d…

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYL322d1/N-Vn

    Plasmid

    #183153

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK/CMV and minP

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. retro-gRNA-mRFP1

    Plasmid

    #112914

    Purpose

    Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

    Depositor

    Christophe Benoist, Diane Mathis

    Article

    Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

    Insert

    mRFP1
    

    Use

    Retroviral

    Promoter

    EF1a

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYL322d1/Vn-C

    Plasmid

    #183154

    Purpose

    Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 alpha 1A AR

    Plasmid

    #45760

    Depositor

    Robert Lefkowitz

    Article

    Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

    Insert

    alpha 1A AR (Adra1a Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187916

    Purpose

    Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. UKNP1.4.2

    Plasmid

    #97364

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. UKNP1.2.2

    Plasmid

    #97357

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-Sa-SauriCas9

    Plasmid

    #135967

    Purpose

    Expresses Sa-SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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