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M31: pHR-SFFVp- TDP-43C::mCherry::SspB

Plasmid

#122669

Purpose

C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

TDP43 (TARDBP Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 218-414

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MET

Plasmid

#116762

Purpose

Lentiviral expression of MET

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MET (MET Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MET

Plasmid

#116762

Purpose

Lentiviral expression of MET

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MET (MET Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E542K

Plasmid

#116479

Purpose

Lentiviral expression of PIK3CA E542K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E542K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E542K

Plasmid

#116479

Purpose

Lentiviral expression of PIK3CA E542K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E542K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDB in pMAX

Plasmid

#120403

Purpose

enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

Insert

EDB-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDB in pMAX

Plasmid

#120403

Purpose

enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

Insert

EDB-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-145

Plasmid

#118529

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-145

Plasmid

#118529

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-MCM4/6

Plasmid

#116951

Purpose

Expresses human MCM4 and human MCM6

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM6 (MCM6 Human)
Human MCM4 (MCM4 Human)

Tags

His6

Expression

Bacterial

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-MCM4/6

Plasmid

#116951

Purpose

Expresses human MCM4 and human MCM6

Depositor

James Chong

Article

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM6 (MCM6 Human)
Human MCM4 (MCM4 Human)

Tags

His6

Expression

Bacterial

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-TEVP

Plasmid

#116062

Purpose

Expresses TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

TEVP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-TEVP

Plasmid

#116062

Purpose

Expresses TEV protease

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

TEVP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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p2K7bsdUBI-mCherry-STIM1

Plasmid

#114178

Purpose

Lentiviral vector for expression of mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry (inserted after signal peptide)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p2K7bsdUBI-mCherry-STIM1

Plasmid

#114178

Purpose

Lentiviral vector for expression of mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry (inserted after signal peptide)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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