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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,022 results for: ENA;

Showing: 7961 - 7980 of 11022 results

  1. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

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  6. Anti-Kv2.1 K+ channel pS586 [L100/1R]

    Plasmid
    #182099
    Purpose
    Mammalian Expression Plasmid of anti-Kv2.1 K+ channel pS586 (Rat). Derived from hybridoma L100/1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Kv2.1 K+ channel pS586 (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182099-plasmid-map-sequence-id-363140

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  9. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  11. Neuregulin-CRD (Cysteine-rich domain, Type III) scFv [N126B/31]

    Plasmid
    #190528
    Purpose
    Mammalian Expression of Neuregulin-CRD (Cysteine-rich domain, Type III) scFV. Derived from hybridoma N126B/31.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant scFV (NRG1 Mouse)
    Use
    Tags
    HA, Sortase, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190528-plasmid-map-sequence-id-376403

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Showing: 7961 - 7980 of 11022 results