We narrowed to 59,446 results for: cloning

1. pAM4937 (pCV0049)

   Plasmid

   #120082

   Purpose

   Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
   

   Use

   Other

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA009

   Plasmid

   #216037

   Purpose

   Fragmid fragment: (C' terminus) Nucleoplasmin NLS

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   NpNLS_v1.1; 3xHA_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ290

   Plasmid

   #129670

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJT040 lenti MCS pEF mIFP WPRE

   Plasmid

   #161929

   Purpose

   Lentiviral expression of mIFP with multiple cloning site upstream the pEF promoter

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   mIFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   pEF

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNP1 eforRed toehold switch

   Plasmid

   #107353

   Purpose

   Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 1 + eforRed
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSF1C-A-RFP

   Plasmid

   #172431

   Purpose

   StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VanGlow-GL

   Plasmid

   #83342

   Purpose

   for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

   Depositor

   Cheng-Yu Lee

   Article

   Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   GFP::Luciferase(nls)

   Expression

   Insect

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR223-BALF5

   Plasmid

   #198577

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF5 (BALF5 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Mutation

   None (wildtype)

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR223-BALF2

   Plasmid

   #198578

   Purpose

   Gateway Entry Clone

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BALF2 (BALF2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Mutation

   None (wildtype)

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ292

    Plasmid

    #129672

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5/FRT/TO V5 DNAJB9

    Plasmid

    #19532

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO GFP DNAJA3

    Plasmid

    #19493

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA3 (DNAJA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAPcrRNA_Lmo

    Plasmid

    #126501

    Purpose

    L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-CreLite

    Plasmid

    #131782

    Purpose

    Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC-P2A-PIF6CreN
    

    Use

    Cre/Lox; Gateway cloning

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5 MEKK4alpha KD

    Plasmid

    #12188

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase dead: K1361M

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAM4951 (pCV0063)

    Plasmid

    #120084

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
    

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNP1 tdTomato toehold switch

    Plasmid

    #107354

    Purpose

    Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 5 + tdTomato
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VanGlow-RR

    Plasmid

    #83343

    Purpose

    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

    Depositor

    Cheng-Yu Lee

    Article

    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    mCherry::Renilla(nls)

    Expression

    Insect

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO HIS HSPH3

    Plasmid

    #19536

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4-like (HSPA4L Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UKNP1.4.1

    Plasmid

    #97363

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge