166,818 results

1. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234445

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_TRC311 ETV1

   Plasmid

   #74981

   Purpose

   Constitutive ETV1 ORF, blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV1 (ETV1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.2_mNeonGreen2

   Plasmid

   #113727

   Purpose

   Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

   Depositor

   Vanessa LaPointe

   Article

   Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

   Insert

   mNeonGreen2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-GAL4

   Plasmid

   #24345

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pMXs-neo

   Plasmid

   #51260

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234446

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAXY0001

   Plasmid

   #179834

   Purpose

   binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

   Insert

   eYGFPuv
   

   Use

   Binary vector for plant transformation

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAB35

   Plasmid

   #175787

   Purpose

   Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

   Depositor

   John Rubinstein

   Article

   Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

   Insert

   SidK (1-278)
   

   Use

   Tags

   3xFLAG and 6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMPP-mCherry-hTRIM21

   Plasmid

   #104972

   Purpose

   Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet1_CylLL_CylM

    Plasmid

    #208759

    Purpose

    Expresses modified cytolysin L (mCylLL) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CylLL
    CylLM
    

    Use

    Tags

    Hisx6 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. human STIM1 CFP

    Plasmid

    #19755

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP Centrin2 (Nigg UK185)

    Plasmid

    #41147

    Depositor

    Erich Nigg

    Article

    Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

    Insert

    Centrin-2 (CETN2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-DJ8R

    Plasmid

    #213974

    Purpose

    AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Neurosci Bull. 2023 Jul 11. doi: 10.1007

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

    Plasmid

    #183775

    Purpose

    AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    NLS-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVTHM-mSox2-3xflag

    Plasmid

    #206376

    Purpose

    Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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