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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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hyeA3A-BE4max

Plasmid

#157944

Purpose

Mammalian expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyeA3A-BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N57G

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-PelB-6H-Ag43-160N-sfGFP

Plasmid

#172886

Purpose

Display sfGFP protein on E. coli cell surface

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

SfGFP
Ag43, partial (flu )

Use

Tags

HHHHHH and TEV recognition site (ENLYFQG)

Expression

Bacterial

Mutation

Deleted 1-160 (N terminal)

Promoter

T7 Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA

Plasmid

#99877

Purpose

Expression of GFP-AURKA in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

AURKA minimal

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IGF1R

Plasmid

#116752

Purpose

Lentiviral expression of IGF1R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-CAMK2A

Plasmid

#23408

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK2A (CAMK2A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-EGFP-2xFYVE

Plasmid

#136996

Purpose

Lentiviral EGFP lipid sensor for PI(3)P

Depositor

Ken-Ichi Takemaru

Insert

EGFP-2xFYVE

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#22900

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K6 only, all other lysines mutated to arginines

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pUCP20T-E2Crimson

Plasmid

#78473

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g3

Plasmid

#80786

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205546

Purpose

Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-ultraID

Plasmid

#207693

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pQTEV-MAT2B

Plasmid

#34795

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

methionine adenosyltransferase II, beta (MAT2B Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(T17N)

Plasmid

#13721

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(T17N) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mCherry (AAV8)

Viral Prep

#114469-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-mCherry (#114469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-mCherry plasmid DNA. CamKIIa-driven expression of mCherry. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

CaMKII

Tags

mCherry

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only