166,818 results

1. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMEXC3H

   Plasmid

   #110080

   Purpose

   Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG-GOI-DE-TurboID-GFP

   Plasmid

   #209389

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTP943

   Plasmid

   #104157

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fab specific nanobody TP886
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDC32-HPB

   Plasmid

   #32078

   Depositor

   Fumi Katagiri

   Article

   Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

   Type

   Empty backbone

   Use

   Gateway destination binary vector

   Tags

   HA-Prescission-Biotin

   Expression

   Mutation

   Promoter

   35S promoter

   Available Since

   Dec. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 PKC delta

   Plasmid

   #8427

   Depositor

   Frederic Mushinski

   Article

   Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

   Insert

   Mouse Protein Kinase C delta (Prkcd Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2_EF1a_TetOn_IRES_mCherry

   Plasmid

   #204155

   Purpose

   Lentivirus backbone expressing rtTA linked to mCherry

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Tet-On 3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NbV5-eGFP

   Plasmid

   #201473

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-SYNZIP17

    Plasmid

    #80671

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP17

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP17
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH41766

    Plasmid

    #48018

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJRA110

    Plasmid

    #209343

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mStayGold fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFLAG_OriM

    Plasmid

    #110097

    Purpose

    Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESC-LEU (V5 and HA)

    Plasmid

    #222486

    Purpose

    Co-expression of two genes in yeast with V5 and HA tags and LEU2 selection

    Depositor

    Kiyofumi Yamada, Tetsuo Matsuzaki

    Article

    Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    HA and V5

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-ADRBK1

    Plasmid

    #23835

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-Arl13b-mCardinal

    Plasmid

    #194542

    Purpose

    Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

    Plasmid

    #217510

    Purpose

    KD Recombinase dependent conditional smGdP-10XV5 cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
    

    Use

    Tags

    smGdP-10XV5

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Promoter

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-IRES-GFP/CREB3L1

    Plasmid

    #214767

    Purpose

    Mammalian expression of human CREB3L1

    Depositor

    Norio Komatsu

    Article

    De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

    Insert

    CREB3L1 (CREB3L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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