168,823 results

1. pMS2-GFP

   Plasmid

   #27121

   Depositor

   Robert Singer

   Article

   Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

   Insert

   MS2
   

   Tags

   EGFP, HA, and SV40 NLS

   Expression

   Mammalian

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156543

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PDCD1 (PDCD1 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYC2085

   Plasmid

   #158721

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDB5050

   Plasmid

   #171126

   Purpose

   A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   OsTIR1
   

   Use

   Other

   Mutation

   non applicable

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156612

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PDCD1LG2 (PDCD1LG2 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PGK plasmid

   Plasmid

   #169744

   Purpose

   Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_LAMP1_HaloTag9

   Plasmid

   #175541

   Purpose

   Lyzosomal expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   LAMP1-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP1337

   Plasmid

   #119270

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_HA1h

   Plasmid

   #190473

   Purpose

   Express the high-affinity histamine sensor GRAB_HA1h in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

   Insert

   Histamine sensor GRAB_HA1h (high affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C9orf72

    Plasmid

    #83437

    Purpose

    Expresses GFP-C9orf72 in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    C9orf72 (C9orf72 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVXCMV100-mNeonGreen-PBD

    Plasmid

    #241366

    Purpose

    Lentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    Pak1 fragment (PAK1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Mutation

    aa 57-141

    Promoter

    Truncated CMV promoter

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-HA_WT POLI

    Plasmid

    #201440

    Purpose

    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-PIK3CA-H1047R

    Plasmid

    #116500

    Purpose

    Lentiviral expression of PIK3CA H1047R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Mutation

    H1047R

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFL-SV40

    Plasmid

    #115352

    Purpose

    firefly luciferase (Fluc) reporter gene driven by SV40 promoter

    Depositor

    Ming-Chih Lai

    Article

    Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-32a(+)-TGFB1

    Plasmid

    #120361

    Purpose

    Produce human recombinant TGFB1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β1 (TGFB1 Human, Synthetic)
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcmv3-IRG1-HA

    Plasmid

    #198181

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1)

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    HA tag is added to C-terminal of target protein

    Promoter

    CMV

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Fis1

    Plasmid

    #182580

    Purpose

    Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Fis1
    

    Tags

    Fis1

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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