We narrowed to 25,719 results for: eng
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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMG059 MBP-CREB-His
Plasmid#154076PurposeExpresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coliDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159845PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-P2A-CIAR_pLenti_CMV_Puro
Plasmid#86503PurposeLenti plasmid for generating CIAR expressing stable cell linesDepositorInsertGFP-P2A-CIAR (SOS1 Human)
UseLentiviralExpressionMammalianMutationT968L in SOScatPromoterCMVAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9 (GB1079)
Plasmid#75399PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
821 pGEX2T PTEN 1-274
Plasmid#10741DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE5max-SF3B1-K700E
Plasmid#200317PurposeExpresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.DepositorInsertsK700E-ng1+pg2
PEmax-2A-MLH1dn
ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR
Plasmid#197029PurposeAAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6DepositorInserthumanized ecDHFR-enOsCas12f1-ecDHFR
UseAAVMutationD52R / T132RPromoterMHCK7, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hMBOAT7-3xFLAG
Plasmid#165474PurposeHuman MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.DepositorInsertMembrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LYRa11-RBD-8h
Plasmid#161822PurposeMammalian expression plasmid for RBD of bat coronavirus isolate LYRa11DepositorInsertReceptor-binding domain (RBD) of spike protein S
TagsGSG linker and 8his tag and HA leader sequenceExpressionMammalianMutationHuman codon optimized RBD (a.a. T324-D522)PromoterCMVAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2-T92Q-Fc(IgG1)
Plasmid#145170PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92QDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1-FL- luciferase
Plasmid#135566PurposeFull Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferaseDepositorInsertFull-length promoter of human retinoic acid receptor-beta gene
PromoterhHuman CYP26A1 gene promoterAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-4A-VN
Plasmid#110647Purposeexpresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR bindingDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
TagsDDK and VNExpressionMammalianMutationC379A, C385A, C421A and C427APromoterCMVAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CCR5
Plasmid#98949PurposeMammalian expression plasmid for N-terminal FLAG-tagged human CCR5DepositorInsertCCR5 (CCR5 Human)
TagsFLAG (dykdddd) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(Q61L)-mCherry
Plasmid#87553PurposePI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1DepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationQ61LPromoterCMVAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)
Plasmid#75412PurposeModule for the expression of the Renilla Luciferase with the silencing suppressor P19DepositorInsertRenilla / P19
UseLuciferase and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pStreptagII 3C EGFR EABR
Plasmid#234994PurposeFor production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminusDepositorAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only
