We narrowed to 59,446 results for: cloning

1. pAT-9208

   Plasmid

   #124221

   Purpose

   Cloning plasmid for a self-targeting gRNA library

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. XW17

   Plasmid

   #65835

   Purpose

   Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Tags

   mCherry

   Expression

   Worm

   Mutation

   The endogenous SphI, KpnI and SacI sites in QF ar…

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

   Plasmid

   #140201

   Purpose

   Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Cloning of crrna an…

   Tags

   dLbCas12a crRNA scaffold

   Promoter

   PgpdA

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO HIS HSPA14

   Plasmid

   #19542

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 14 (HSPA14 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO HIS DNAJA3

   Plasmid

   #19547

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. UKNP.1.12.1

   Plasmid

   #97384

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. UKNP1.21.2

   Plasmid

   #98200

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQTEV-MAPRE1

   Plasmid

   #31589

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTL-Bag3-gRNA3

   Plasmid

   #196254

   Purpose

   Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTL-Bag2-gRNA2

    Plasmid

    #196252

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTL-Bag1-gRNA1

    Plasmid

    #196251

    Purpose

    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTL-Bag4-gRNA4

    Plasmid

    #196255

    Purpose

    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. UKNP1.19.2

    Plasmid

    #98193

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid

    #48992

    Purpose

    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Inserts

    cre recombinase
    Luciferase
    

    Use

    Luciferase; Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    Unknown

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. FB027

    Plasmid

    #119712

    Purpose

    Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBEG R2-iPuro-L3 #BGV205

    Plasmid

    #48987

    Purpose

    Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Insert

    puromycin resistance
    

    Use

    Gateway cloning vector

    Tags

    IRES (weak)

    Promoter

    NA

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO DNAJB2a

    Plasmid

    #19469

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2a (DNAJB2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sha2Cas9-HF

    Plasmid

    #192142

    Purpose

    Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Sha2Cas9-HF (R247A and N415A)

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Sa-SchCas9

    Plasmid

    #192137

    Purpose

    Expresses Sa-SchCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

    Insert

    Sa-SchCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQTEV-GTF2F2

    Plasmid

    #31323

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    general transcription factor IIF (GTF2F2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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