We narrowed to 15,951 results for: grna

1. pXFokI-dCas9

   Plasmid

   #60901

   Purpose

   Dual Expression Vector for FokI-dCas9 and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG3B-U3Ub

   Plasmid

   #134750

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA- OsU3, zCas9-Ubi1

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU1/Sm/3' Box_(GLuc)_TOP1

   Plasmid

   #68437

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. psAAVS1-T2

   Plasmid

   #60543

   Purpose

   GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

   Depositor

   William Simonds

   Article

   Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

   Insert

   ProtoSpacer AAVS1-T2 (AAVS1 Human)
   

   Use

   CRISPR; Kan

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE8

   Plasmid

   #79463

   Purpose

   sgRNA shuttle vector; module 2E

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV/MASC_(GLuc)_INT

   Plasmid

   #68440

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTX198

   Plasmid

   #89262

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsPDS-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Maize Ubiquitin1 promoter

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC523

    Plasmid

    #62320

    Purpose

    sgRNA for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGE12

    Plasmid

    #79469

    Purpose

    sgRNA shuttle vector; module 5

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE13

    Plasmid

    #79471

    Purpose

    sgRNA shuttle vector; module 6

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE14

    Plasmid

    #79473

    Purpose

    sgRNA shuttle vector; module 7

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE15

    Plasmid

    #79474

    Purpose

    sgRNA shuttle vector; module 8E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGE10

    Plasmid

    #79466

    Purpose

    sgRNA shuttle vector; module 4

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ141C2.0

    Plasmid

    #99905

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGE5

    Plasmid

    #79461

    Purpose

    sgRNA shuttle vector; module 1

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge