We narrowed to 7,375 results for: ef1a

1. pLv-EF1a-Parkin(S65A)-A92mKO2

   Plasmid

   #213557

   Purpose

   Lentiviral vector for stable expression of S65A Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S65A mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLv-EF1a-Parkin(N273K)-A92mKO2

   Plasmid

   #213555

   Purpose

   Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N273K mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLv-EF1a-Parkin(W403A)-A92mKO2

   Plasmid

   #213558

   Purpose

   Lentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   W403A mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

   Plasmid

   #204620

   Purpose

   Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ef1a-DIO-eGCaMP(plus)

   Plasmid

   #201149

   Purpose

   AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

   Depositor

   Andre Berndt

   Article

   Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

   Insert

   eGCaMP+
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184690

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-ArgSen (-)P2A (-)NLS

   Plasmid

   #184699

   Purpose

   Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   ArgSen (-)P2A (-)NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-fDIO-tdTomato-WPRE

    Plasmid

    #187112

    Purpose

    FLP-dependent expression of tdTomato under EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Hygro-EF1A-RG-Linker-eGFP

    Plasmid

    #176071

    Purpose

    EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    RG
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-Hygro-EF1A-KR-Linker-eGFP

    Plasmid

    #176066

    Purpose

    EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    KR
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

    Plasmid

    #170249

    Purpose

    Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G23V

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-EF1a-KIF5AR280H-HA-IRES-Puro

    Plasmid

    #166953

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A-R280H (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    KIF5A-R280H

    Promoter

    eF1a

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM466 EF1a VP16-ZF43 (TUPV2)

    Plasmid

    #138730

    Purpose

    mMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF43
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-3NLS-Klf4 TALE-GCN4

    Plasmid

    #120546

    Purpose

    Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

    Depositor

    Yu Wang

    Article

    Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

    Insert

    NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

    Plasmid

    #110159

    Purpose

    Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

    Insert

    TFAP4 (transcription factor AP-4) (TFAP4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E135A/S139A

    Promoter

    CMV

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

    Plasmid

    #69584

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHAGE-EF1a MICA*009-IRES-ZsGreen

    Plasmid

    #114007

    Purpose

    Induces expression of human MICA allele 009 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICA*009 (MICA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

    Plasmid

    #134867

    Purpose

    Expresses EGFP-tagged nuclear envelope marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-EF1a-MICB*005-IRES-ZsGreen

    Plasmid

    #114008

    Purpose

    Induces expression of human MICB allele 005 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICB*005 (MICB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-EF1a-HIF2a dPA (P405A, P531A)

    Plasmid

    #232954

    Purpose

    Stably express a constitutively stable version of HIF2a (HIF2a dPA)

    Depositor

    William Kaelin

    Article

    Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

    Insert

    HIF2a (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P405A, P531A

    Promoter

    EF1a

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204189

    Purpose

    Doxycycline inducible lentiviral vector for human GATA6 overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJRH-1363 LV EF1a-CD19 IRES-EGFP

    Plasmid

    #201919

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid

    #108542

    Purpose

    Lentivirus vector expressing Luciferase with blasticidin resistance

    Depositor

    Javier Alcudia

    Inserts

    Luciferase
    Neomycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1apha and PGK

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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