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AAEL006076 AstAR

Plasmid

#115381

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL006076 AstAR (AaeL_AAEL006077 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL006636 DSK R

Plasmid

#115392

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL006636 DSK R (LOC5574266 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LOC110678930 NPY receptor

Plasmid

#115379

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC110678930 NPY receptor (LOC110678930 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL017313 GPCR

Plasmid

#115404

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL017313 GPCR (LOC23687733 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL003473 MIP R

Plasmid

#115398

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL003473 MIP R (LOC5578188 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL012920 AstC R

Plasmid

#115390

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL012920 AstC R (LOC5577003 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL009024 PDF R

Plasmid

#115408

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL009024 PDF R (LOC5571400 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HM110 prp16-2

Plasmid

#111277

Purpose

prp16-2 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Madhani et al Genetics. 1994 Jul;137(3):677-87.

Insert

PRP16

Expression

Yeast

Mutation

prp-2 (D575N)

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1355

Plasmid

#108994

Purpose

generic knockin vector for C-terminal tagging with GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

GFP

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 S1338A

Plasmid

#111808

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (TSC2 Fly)

Tags

3xFlag

Expression

Insect

Mutation

S1338A

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_4

Plasmid

#102966

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_4 (NFS1 Human)

Use

Lentiviral and RNAi

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663K

Plasmid

#92239

Purpose

Express R663K mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663K mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Lysine

Promoter

T7 promoter

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_3

Plasmid

#102965

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_3 (NFS1 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-W695del

Plasmid

#92241

Purpose

Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Tryptophan 695 deleted

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663Q

Plasmid

#92240

Purpose

Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663Q mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Glutamine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-H698A

Plasmid

#92237

Purpose

Express H698A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-H698A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Histidine 698 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-R663A

Plasmid

#92238

Purpose

Express R663A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-R663A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Arginine 663 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 E235A

Plasmid

#87601

Purpose

Encodes Prp5 E235A for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E235A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 N399D

Plasmid

#87602

Purpose

Encodes Prp5 N399D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N399D

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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