We narrowed to 26,617 results for: multi
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Plasmid#130692PurposeLentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.DepositorInserthuman OCT4 (POU5F1 Human)
UseLentiviralAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-i53-bpA-EF1BFP
Plasmid#111145PurposeExpression of i53 and a BFP reporter in mammalian cellsDepositorExpressionMammalianMutationhuman Ubiquitin, contains 8 point mutationsPromoterCAG promoter and EF1Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIH-mRuby2-BAX
Plasmid#111628PurposeFluorescent fusion protein used to visualise mouse BAX, with hygromycin selectionDepositorAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-RhoA Q63L
Plasmid#81058Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
ExpressionBacterialAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC638
Plasmid#62283PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationNuclease activity has been deactivated by mutatio…PromoterpAdh and pGal10Available SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2
Plasmid#51250PurposeEncodes N-3xHA-TEV-mouseGli2-CDepositorAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)
Plasmid#47870PurposeAll-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.DepositorInsertsNmCas9
U6pr-tracrRNA
OCT4 crRNA
UseCRISPRTagsHA and NLSExpressionMammalianAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRenilla
Plasmid#118059PurposeGBPart - Renilla Luciferase CDSDepositorInsertRenilla
UseLuciferase and Synthetic Biology; GbpartAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p(A)n-PAUSE
Plasmid#118068PurposeTranscription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin geneDepositorInsertSynthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPac0
Plasmid#12094DepositorTypeEmpty backboneExpressionInsectAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
tetO-Bcl2-IRES-tdtomato(TET005)
Plasmid#117857PurposeThis plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.DepositorAvailable SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR(T/A)
Plasmid#118463PurposeNegative-control mutant for ExRai-AktAR biosensor.DepositorInsertExRai-AktAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC147-pCR8-dCas9VP64
Plasmid#48219PurposedCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP64MutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralPromoterEFSAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ABE-NT-sgRNA
Plasmid#112734PurposeAAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNADepositorInsertABE-NT-sgRNA
UseAAVAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132A2.0
Plasmid#99887PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterAtU6Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCORE-UH
Plasmid#72239PurposeContains the CORE cassette with KlURA3 and hyg markersDepositorInsertsKlURA3
hyg
ExpressionBacterialAvailable SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only