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pLKO-Rabep1 shRNA 369958-CMV-TurboGFP

Plasmid

#254777

Purpose

Rabep1 shRNA 369958 in pLKO backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 (RABEP1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Gcat1-guides

Plasmid

#254767

Purpose

Gcat1 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Gcat1 (gcat Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆N∆CC-G pc5089

Plasmid

#229765

Purpose

Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-C∆CC-G pc5091

Plasmid

#229766

Purpose

Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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U6-miR.FF4

Plasmid

#235256

Purpose

Non-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FF4 hairpin

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-5

Plasmid

#221491

Purpose

NKX2-5 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 D90A

Plasmid

#183641

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 90 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 20A

Plasmid

#183639

Purpose

Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

Plasmid

#169743

Purpose

CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

mScarlet-I

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc IRESv24

Plasmid

#141087

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEPLuc

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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D74_Ld_291730

Plasmid

#135887

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

endosomal integral membrane protein, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOGGUSPlus

Plasmid

#64400

Purpose

Oat globulin Glo1 promoter driving expression of GUSPlus

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Tags

GUSplus (modified Staphylococcus sp. b-glucuronid…

Expression

Plant

Promoter

Oat globulin Glo1

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
Tol2-LyzC-delta5_2 Rabep1-T2A-RFP-Caax

Plasmid

#254768

Purpose

Neutrophil specific Rabep1 truncation lacking rab5 binding domain in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (RABEP1 Human)

Use

Zebrafish expression

Mutation

5/2 deletion

Promoter

Lyzc

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-Rabep1 shRNA 369958-CMV-FL Rabep1

Plasmid

#254779

Purpose

Rabep1 shRNA 369958 with expression of full length Rabep1 rescue in pLKO backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 and Rabep1 (RABEP1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-U6ac-Praf2-guides

Plasmid

#254765

Purpose

Praf2 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Praf2 (praf2 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-delta4_2 Rabep1-T2A-RFP-Caax

Plasmid

#254769

Purpose

Neutrophil specific Rabep1 truncation lacking rab4 binding domain in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (RABEP1 Human)

Use

Zebrafish expression

Mutation

4/2 deletion

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Arhgap20-guides

Plasmid

#254763

Purpose

Arhgap20 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Arhgap20 (arhgap20 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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