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We narrowed to 16,015 results for: grn

Showing: 8301 - 8320 of 16015 results

  1. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid
    #68424
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68424-plasmid-map-sequence-id-125555

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  2. pAGK112

    Plasmid
    #228127
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228127-plasmid-map-sequence-id-455412

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  3. MSP2262

    Plasmid
    #70703
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A & H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70703-plasmid-map-sequence-id-127765

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  5. pAH243

    Plasmid
    #121147
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Mutation
    Promoter
    S.pombe rrk1 and nmt41
    Available Since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121147-plasmid-map-sequence-id-234831

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  7. pCMV/3' Box_(GLuc)_INT

    Plasmid
    #68436
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68436-plasmid-map-sequence-id-120214

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  14. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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Showing: 8301 - 8320 of 16015 results