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We narrowed to 63,052 results for: cloning

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Showing: 8321 - 8340 of 63052 results

  1. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

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  2. MZB117

    Plasmid
    #228481
    Purpose
    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    GST (glutathione S-transferase)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228481-plasmid-map-sequence-id-454022

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  3. Topaz-C1

    Plasmid
    #54744
    Purpose
    Localization: C1 Cloning Vector, Excitation: 514, Emission: 527
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Topaz
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. JRed-C1

    Plasmid
    #54788
    Purpose
    Localization: C1 Cloning Vector, Excitation: 584, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    JRed
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. JRed-N1

    Plasmid
    #54789
    Purpose
    Localization: N1 Cloning Vector, Excitation: 584, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    JRed
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. AsRed2-N1

    Plasmid
    #54703
    Purpose
    Localization: N1 Cloning Vector, Excitation: 576, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AsRed2
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Publication
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    210212-plasmid-map-sequence-id-416400

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  11. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62343-plasmid-map-sequence-id-102266

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  14. HcRed1-CytERM-N-17

    Plasmid
    #55840
    Purpose
    Localization: N1 Cloning Vector, Excitation: 588, Emission: 618
    Depositor
    Michael Davidson
    Insert
    CytERM
    Tags
    HcRed1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55840-plasmid-map-sequence-id-221613

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  15. pICSL13008

    Plasmid
    #245656
    Purpose
    Level 0 Golden Gate part Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245656-plasmid-map-sequence-id-495550

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  16. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104910-plasmid-map-sequence-id-206061

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  17. p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

    Plasmid
    #214462
    Purpose
    Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PIP5K1A (PIP5K1A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214462-plasmid-map-sequence-id-425204

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  18. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid
    #214464
    Purpose
    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-RIOK1 (RIOK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214464-plasmid-map-sequence-id-423214

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  19. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

    Plasmid
    #214459
    Purpose
    Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214459-plasmid-map-sequence-id-423212

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Showing: 8321 - 8340 of 63052 results