Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,227 results for: sgRNA

Clear keywords
Showing: 8321 - 8340 of 9227 results

  10. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

    Enlarge

  11. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

    Enlarge

  17. pPHAGE-CR-PINK1-C-TAP

    Plasmid
    #128510
    Purpose
    Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.
    Depositor
    Mohan Babu
    Publication
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    PTEN induced kinase 1 (PINK1 Human)
    Use
    Lentiviral
    Tags
    FLAG and HA tags
    Expression
    Mammalian
    Mutation
    Synonymous silent mutation in Q5 to render sgRNA …
    Promoter
    CMV
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    128510-plasmid-map-sequence-id-256209

    Enlarge

  18. pTBL680 CHYRON3 integration construct

    Plasmid
    #126448
    Purpose
    To integrate the CHYRON3 locus at AAVS1 in human cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    Expression
    Mammalian
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    CMV and human U6/3xLacO
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126448-plasmid-map-sequence-id-246061

    Enlarge

  19. CRISPRecise Kit

    Plasmid kit
    #1000000235
    Purpose
    Set of high-fidelity SpCas9 variants that can be used to efficiently edit targets with practically no detectable off-target effects.
    Depositor
    Ervin Welker
    Application
    Genome Editing
    Vector type
    Mammalian Expression
    Editing type
    CRISPR
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8321 - 8340 of 9227 results