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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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PB-PE iSM

Plasmid

#235995

Purpose

Expresses iSM (iSpyMac) prime editor and pegRNA in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

bpSV40 NLS

Expression

Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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iSM_PPL2-IP

Plasmid

#235989

Purpose

Expresses iSM_PPL2 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_PPL2

Use

CRISPR

Expression

Mammalian

Mutation

replacement of 5 amino acids of Smac-Cas9 PL2 wit…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-N2-BE4-puro

Plasmid

#226583

Purpose

Express N2-BE4 base editor in mammalian cells

Depositor

Gang Wang

Article

Zhao et al Nat Commun. 2024 Sep 17;15(1):8140. doi:

Insert

N2-BE4

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM2_pGEX

Plasmid

#228799

Purpose

Recombinant expression of mouse MSI1 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_2xRRM_pGEX

Plasmid

#228800

Purpose

Recombinant expression of MSI2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM1_pGEX

Plasmid

#228801

Purpose

Recombinant expression of MSI2 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM1 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI2_RRM2_pGEX

Plasmid

#228802

Purpose

Recombinant expression of MSI2 RRM2 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-2 RRM2 (MSI2 Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_pGEX_noSBP

Plasmid

#228803

Purpose

Recombinant expression of UNK with mono-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt (UNK Human)

Tags

GST and HIS

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Swap_pGEX

Plasmid

#228804

Purpose

Recombinant expression of UNK Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6, 1-3 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_Dual_pGEX

Plasmid

#228805

Purpose

Recombinant expression of UNK Dual with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Unkempt Zinc Fingers 4-6 x2 (UNK Human)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_2xRRM_pGEX_Flag_noSBP

Plasmid

#228796

Purpose

Recombinant expression of mouse MSI1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 (Msi1 Mouse)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_SWAP_2xRRM_pGEX

Plasmid

#228797

Purpose

Recombinant expression of mouse MSI1 Swap with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM2, RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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MSI1_RRM1_pGEX

Plasmid

#228798

Purpose

Recombinant expression of mouse MSI1 RRM1 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al J Biol Chem. 2025 May;301(5):108435. doi

Insert

Musashi-1 RRM1 (Msi1 Mouse)

Tags

GST and SBP

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-Myo10 ΔF

Plasmid

#145139

Purpose

Expresses mScarlet-I tagged MYO10 construct with a MyTH/FERM domain deletion

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 ΔF (MYO10 Human)

Expression

Mammalian

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FERM (Myo10)

Plasmid

#145140

Purpose

Expresses EGFP-tagged MyTH/FERM domains of MYO10.

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MyTH/FERM domains of MYO10 (MYO10 Human)

Expression

Mammalian

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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