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We narrowed to 62,316 results for: cloning

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Showing: 8341 - 8360 of 62316 results

  12. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Article
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    pHluorin-mKate2 fusion
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

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  13. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  14. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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Showing: 8341 - 8360 of 62316 results