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Kv2.1 K+ channel scFv [K39/25]

Plasmid

#182067

Purpose

Mammalian Expression of Kv2.1 K+ channel scFv. Derived from hybridoma K39/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Kv2.1 K+ channel (Rattus norvegicus) (Kcnb1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GIT1 [N39B/8R]

Plasmid

#177545

Purpose

Mammalian Expression Plasmid of anti-GIT1 (Rat). Derived from hybridoma N39B/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GIT1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Git1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

Plasmid

#169992

Purpose

Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

TEV-siriusGFP-FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-Puma-pEGFP-C1

Plasmid

#166739

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Puma (BBC3 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Article

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Expression

Mammalian

Promoter

RSV promoter

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3C G126

Plasmid

#123096

Purpose

Expresses 3xFLAG-LC3C G126 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-29Q

Plasmid

#84350

Purpose

Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp2-a del.C2AB

Plasmid

#40059

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.C2AB (SYTL2 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of C2A and C2B domains (601-910)

Promoter

Tac

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-S35

Plasmid

#39517

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97deltaD2(K251A)

Plasmid

#17230

Depositor

Hemmo Meyer

Article

Ramadan et al Nature. 2007 Dec 20. 450(7173):1258-62.

Insert

p97 (Vcp Mouse)

Tags

His

Expression

Bacterial

Mutation

change codon 457 to stop codon, change lysine 25…

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCHC036 (∆pHG1)

Plasmid

#226206

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for the pHG1 megaplasmid replication region

Use

Synthetic Biology

Mutation

∆helD ∆repA ∆repB ∆parAB

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDF-hLPYK

Plasmid

#220231

Purpose

Inducible expression of human liver pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Human liver pyruvate kinase (PKLR Human)

Expression

Bacterial

Promoter

T7

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00652

Plasmid

#39713

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11799 (fd68A Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only