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TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium OPEN Accessory Reagent Set
Plasmid Kit#1000000013PurposeContains plasmids and strains used to engineer Zinc Finger Nucleases (ZFNs) via the Oligomerized Pool ENgineering (OPEN) method.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-G-NG
Plasmid#220897PurposeExpress haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-G-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-G
Plasmid#220894PurposeExpress haA3A-CBE-G (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-G
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-haA3A-CBE-G-N
Plasmid#220898PurposeAAV genome encoding the N-terminal of haA3A-CBE-GDepositorInserthaA3A-CBE-G-N
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-haA3A-CBE-G-C
Plasmid#220899PurposeAAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNADepositorInserthaA3A-CBE-G-C
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A-NG
Plasmid#220895PurposeExpress haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-A-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-A
Plasmid#220892PurposeExpress haA3A-CBE-A (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA
Plasmid#220893PurposeExpress haA3A-CBE-VA (with SpCas9) in mammalian cellsDepositorInserthaA3A-CBE-VA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-haA3A-CBE-VA-NG
Plasmid#220896PurposeExpress haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cellsDepositorInserthaA3A-CBE-VA-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-DsRed(isoform 2)
Plasmid#99563Purposemammalian expression of TMEM230-dsRed (isoform 2)DepositorInsertTMEM230 (TMEM230 Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-pEGFP(isoform 2)
Plasmid#99562Purposemammalian expression of TMEM230-GFP (isoform 2)DepositorInsertTMEM230 (TMEM230 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-pEGFP(isoform 1)
Plasmid#99561Purposemammalian expression of TMEM230-GFP (isoform 1)DepositorInsertTMEM230 (TMEM230 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-IRES(isoform 2)
Plasmid#99564Purposemammalian expression of TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-WT-IRES(isoform 1)
Plasmid#99558Purposemammalian expression of TMEM230 (isoform 1) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p026 pCS2-EnR
Plasmid#11028DepositorInsertengrailed (en Fly)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationEngrailed repressor domain (bases encoding amino …PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG-Brn2-DBD-EnR
Plasmid#19714DepositorUseTagsExpressionMammalianMutationmouse Brn2 DNA binding domain-Drosophila engraile…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M4/X184PG-IRES(isoform 2)
Plasmid#99568Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM4/X184PGPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M3/Y92C-IRES(isoform 2)
Plasmid#99567Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM3/Y92CPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M2/X184W-IRES(isoform 2)
Plasmid#99566Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM2/X184WPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M1/R141L-IRES(isoform 2)
Plasmid#99565Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM1/R141LPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M2/X184W-IRES(isoform 1)
Plasmid#99560Purposemammalian expression of mutant TMEM230 (isoform 1) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM2/X184WPromoterCMVAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M1/R141L-IRES(isoform 1)
Plasmid#99559Purposemammalian expression of mutant TMEM230 (isoform 1) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM1/R141LPromoterCMVAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSin-5HT-1.0
Plasmid#172882Purposemake sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cellsDepositorInsert5HT SENSOR
UseTagsExpressionMammalianMutationPromotersp6Available sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas ServiceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Lb
Plasmid#138096PurposeGolden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Lb
Plasmid#138105PurposeGolden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Lb
Plasmid#138102PurposeGolden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
