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Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CMV/WPREΔU3
Plasmid#190726PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b motor
Plasmid#187364PurposeExpresses rat myosin 1b isoform b motor domain labelled with EGFPDepositorInsertmyosin 1b isoform b motor domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b-Tail
Plasmid#187365PurposeExpresses rat myosin 1b isoform b Tail domain labelled with EGFPDepositorInsertmyosin 1b isoform b Tail domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK73_htz-1
Plasmid#174546Purposehtz-1 targeting gRNA expressionDepositorInserthtz-1 targeting gRNA
UseTagsExpressionWormMutationPromoterCe-U6 promoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJet-gR-X4ex2-DTS
Plasmid#177154PurposeDonor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4DepositorInsertMT-C34-pA with CXCR4 arms of homology
UsePcr cloningTagsExpressionMutationPromoternoneAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-ratCENP-C
Plasmid#174445Purposein vitro transcription template for rat CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-pumilioCENP-C
Plasmid#174446Purposein vitro transcription template for R. pumilio CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-H2b-emiRFP670-P2A-TEVP
Plasmid#174379PurposeAAV virus for expressing TEVP and H2b-emiRFP670DepositorInsertTEVP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRV∆G-NPEST-4Cre
Plasmid#174380PurposeRabies vector for expressing Cre recombinase and N gene with PEST domainDepositorInsertCre recombinase
UseRabiesTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB204: UbC-GFP-Tg_SAP30L-WPRE
Plasmid#168109PurposeLentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFPDepositorInsertSAP30L (SAP30L T. guttata)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterUbCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA844 - hrp-1p::hrp-1mScarlet
Plasmid#139204PurposeExpresses mScarlet tagged hrp-1DepositorInserthrp-1 (hrp-1 Nematode)
UseTagsExpressionWormMutationmScarlet added between RRMs and LCPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
UseTags6xHisExpressionBacterialMutationPromoterrbcS-SH1: T7, chaperones: T13Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
UseTagsExpressionBacterialMutationPromoterrbcS-T4a: T7, chaperones: T10Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K30A
Plasmid#105694PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K30A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 30 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K31A
Plasmid#105695PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K31A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 31 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_C21A
Plasmid#105697PurposeBacterial expression plasmids for production of recombinant metallothionein 2a C21A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationCysteine 21 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_K20A
Plasmid#105698PurposeBacterial expression plasmids for production of recombinant metallothionein 2a K20A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationLysine 20 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT2a_S6A
Plasmid#105699PurposeBacterial expression plasmids for production of recombinant metallothionein 2a S6A mutantDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationSerine 6 to AlaninePromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.2
Plasmid#139479PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
UseTagsExpressionMammalianMutationV554LPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0649
Plasmid#129010PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET20b-YbAnbu
Plasmid#129637PurposeExpression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)DepositorInsertYbAnbu
UseTagsHis6-tagExpressionBacterialMutationV14A and G107HPromoterT7Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET20b-YbAnbuT1A
Plasmid#129638PurposeExpression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)DepositorInsertYbAnbuT1A
UseTagsHis6-tagExpressionBacterialMutationT1A, V14A and G107HPromoterT7Available sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0655
Plasmid#129016PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0654
Plasmid#129015PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0653
Plasmid#129014PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporterDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0650
Plasmid#129011PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0648
Plasmid#129009PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0651
Plasmid#129012PurposeControl for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporterDepositorInsert5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only