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Hv_HPT:pCSRM2C:Gus_introns

Plasmid

#215191

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Scream2

Promoter

BdSCRM2

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN13-Elen0616-Cber(ElDSM2243)

Plasmid

#225308

Purpose

Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cber

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA-MS2

Plasmid

#207624

Purpose

Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

crRNA-MS2

Use

CRISPR

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-MCP

Plasmid

#207626

Purpose

Expresses LambdaN-MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ALFA-MCP

Plasmid

#207628

Purpose

Expresses ALFA-tagged MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

ALFA-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMulti_K_ccdB

Plasmid

#223852

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_A_ccdB

Plasmid

#223851

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET27b+R

Plasmid

#188245

Purpose

Empty backbone vector for protein expression in Escherichia coli and his-tag purification

Depositor

Yiping Qi

Article

Fang et al Methods Mol Biol. 2023;2653:153-171. doi

Type

Empty backbone

Tags

6xHis tag and TEV site

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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