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We narrowed to 16,604 results for: LEA

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Showing: 8541 - 8560 of 16604 results

  1. PB-Neo-TetO-Cdk13_Nterm_FlagHa

    Plasmid
    #127345
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.
    Insert
    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Base pairs 1-1554 of transgene are codon optimize…
    Promoter
    TetO Promoter
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    127345-plasmid-map-sequence-id-246535

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  2. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid
    #196887
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    196887-plasmid-map-sequence-id-442624

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  3. hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP

    Plasmid
    #210509
    Purpose
    expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Inserts
    hSyn promoter (RIC8B )
    PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210509-plasmid-map-sequence-id-415577

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  5. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  6. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid
    #185484
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185484-plasmid-map-sequence-id-363406

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  7. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

    Plasmid
    #185486
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185486-plasmid-map-sequence-id-362672

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  8. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid
    #185487
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185487-plasmid-map-sequence-id-362677

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  9. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  10. t1-405(del30)

    Plasmid
    #166128
    Purpose
    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del30) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, with 30 amino acid deletion in th…
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166128-plasmid-map-sequence-id-330260

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  11. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  20. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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Showing: 8541 - 8560 of 16604 results