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pEMS2044

Plasmid

#79652

Purpose

ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple255-EmGFP WPRE

Use

AAV

Promoter

PAX6 HS234Z Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

Plasmid

#160616

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Frt-hspB4

Plasmid

#63095

Purpose

Expression of HSPB4 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb4 (CRYAA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-NEMO-C54/347A

Plasmid

#27268

Depositor

Thomas Gilmore

Article

Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

Insert

NEMO (IKBKG Human)

Tags

FLAG

Expression

Mammalian

Mutation

Cys54Ala Cys347Ala

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pEMS2049

Plasmid

#79657

Purpose

ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple260-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTmycEIF2C1

Plasmid

#19871

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pEMS2045

Plasmid

#79653

Purpose

ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple256-EmGFP WPRE

Use

AAV

Promoter

PAX6 enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Promoter

PAX6 MiniPromoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2043

Plasmid

#79651

Purpose

ssAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple254-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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ARB365

Plasmid

#124048

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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