We narrowed to 4,411 results for: ado

1. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

   Plasmid

   #140168

   Purpose

   Expresses a regulatable secretory cargo for Tetrahymena thermophila

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   MTT1

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3789 pmko-neo RNAi-GFP

   Plasmid

   #8881

   Depositor

   Stanley Korsmeyer

   Article

   Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

   Insert

   GFP
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pEF-pIgH-ESCargo(FTV)

   Plasmid

   #140160

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLM-fSV2A

   Plasmid

   #27512

   Depositor

   Michel Sadelain

   Article

   Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

   Insert

   OCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK01_PalkS_alkS

   Plasmid

   #166502

   Purpose

   Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

   Depositor

   Sven Panke

   Article

   Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

   Insert

   alkS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   L401G

   Promoter

   PalkS

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

   Plasmid

   #176630

   Purpose

   Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC103-PBNeo-U6-sgRNA-Expression

   Plasmid

   #48228

   Purpose

   sgRNA expression on PiggyBac vector with Neo-selectable marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FsC_pET29b

   Plasmid

   #215405

   Purpose

   Expression construct for FsC gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   FsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-FLAG-hEcto/Tif1g

   Plasmid

   #20902

   Depositor

   Stefano Piccolo

   Article

   Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

   Insert

   Ectodermin (TRIM33 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N-terminal deletion of 37 aminoacids; Ectodermin …

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-GGPPipi

    Plasmid

    #53280

    Purpose

    Contains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Dlx-tTA

    Plasmid

    #210730

    Purpose

    AAV vector expressing tetracycline-controlled transactivator (tTA) under Dlx promoter (for expressing tTA in GABAergic neurons)

    Depositor

    Wei Xu

    Article

    Thompson et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    tetracycline-controlled transactivator (tTA)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Dlx

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRN3P_T3_ABEmax_IVT

    Plasmid

    #171761

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    ABEmax
    

    Use

    Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRN3P_T3_BE4_IVT

    Plasmid

    #171762

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE4
    

    Use

    Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRII p15

    Plasmid

    #16454

    Depositor

    Bert Vogelstein

    Article

    Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

    Insert

    p15 (CDKN2B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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