We narrowed to 22,630 results for: CRISPR

1. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

   Plasmid Kit

   #1000000062

   Purpose

   Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

   Depositor

   Takashi Yamamoto

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

2. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

   Plasmid Kit

   #1000000154

   Purpose

   Vectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.

   Depositor

   Jeffrey Essner

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

3. FR-E01

   Bacterial Strain

   #118727

   Purpose

   Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

   Bacterial Resistance

   None

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

4. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

   Plasmid Kit

   #1000000210

   Purpose

   Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

   Depositor

   Vincent Martin

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

5. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Johannes Stuttmann, Sylvestre Marillonnet

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

6. AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)

   Viral Prep

   #107312-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Promoter

   Tags

   mCherry (physically separate, not a fusion protein)

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

   Viral Prep

   #112677-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

   Viral Prep

   #112677-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

   Viral Prep

   #112677-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral Prep

    #112677-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

    Viral Prep

    #112677-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

12. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral Prep

    #112677-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

13. Corynebacterium glutamicum::RFP

    Bacterial Strain

    #79642

    Purpose

    Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Bacterial Resistance

    Gentamicin

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

15. bMS.346

    Bacterial Strain

    #220588

    Purpose

    Increase editing efficiency of retron and oligo recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

16. LC-E02

    Bacterial Strain

    #78551

    Purpose

    E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

17. LC-E03

    Bacterial Strain

    #78552

    Purpose

    E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Bacterial Resistance

    None

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pGMC00010 (aka pRC0495)

    Plasmid

    #195295

    Purpose

    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGMC00009 (aka pRC0494)

    Plasmid

    #195294

    Purpose

    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEC1.2/EC1.1_spCas9_GFP

    Plasmid

    #161933

    Purpose

    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

    Depositor

    Christian Fankhauser

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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