We narrowed to 24,829 results for: crispr

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

Plasmid

#188768

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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BE4

Plasmid

#100802

Purpose

Expresses BE4 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ203 (pMDC32-Ubi1)

Plasmid

#86207

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance marker

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Promoter

Maize ubiquitin 1 (ZmUbi1)

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Nterm-PE2max

Plasmid

#198734

Purpose

AAV genome encoding N-terminal PE2max

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

Cbh

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)

Pooled Library

#1000000049

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Feng Zhang

Available Since

May 8, 2014

Availability

Academic Institutions and Nonprofits only
pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-p15

Plasmid

#62656

Purpose

Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

Depositor

Kristala Prather

Article

Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

Insert

exo, beta, gam, sgRNA

Expression

Bacterial

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Mouse sgRNA library Brie in lentiCRISPRv2

Pooled Library

#73632

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)

Pooled Library

#1000000111

Purpose

Genome-wide CRISPR gRNA pooled library for activation of human genes

Depositor

John Doench, David Root

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only