Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)

For expression of toxic DddA

Increase editing efficiency of retron and oligo recombineering

E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.

Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP

Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP

CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection

aTc inducible OptiCas9

cloning of a sgRNA

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, hygromycin and red fluorescent seed selection)

Expression of CRISPR-associated transposases

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, kanamycin and red fluorescent seed selection)

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)

CRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)