We narrowed to 5,835 results for: pCas

1. B-HeFSpCas9-A695Q

   Plasmid

   #207368

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A695Q
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9-A926Q

   Plasmid

   #207370

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A926Q
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-evoSpCas9-E526K

   Plasmid

   #207365

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-E526K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-evoSpCas9-V495M

   Plasmid

   #207363

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-V495M
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Y515N, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-HiFi SpCas9

   Plasmid

   #207362

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R691A, amino acids 1005-1013 replaced with two gl…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cav2.2 HA pCAGGS

   Plasmid

   #206052

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Promoter

   CMV/B-actin

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. B-HeFSpCas9-A1060R

   Plasmid

   #207372

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1060R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B-HeFSpCas9-A661R

    Plasmid

    #207367

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. B-evoSpCas9-Q661R

    Plasmid

    #207366

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-Q661R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. B-evoSpCas9-N515Y

    Plasmid

    #207364

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-N515Y
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. HypaR-SpCas9

    Plasmid

    #126757

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL0_32 [Pcat]

    Plasmid

    #198945

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    Pcat
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL0_51 [PcatA]

    Plasmid

    #198964

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    PcatA
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ATG101

    Plasmid

    #189590

    Purpose

    To make ATG101/ATG13 HORMA complex

    Depositor

    James Hurley

    Article

    Shi et al J Cell Biol. 2020 Jul 6;219(7):e20191104

    Insert

    ATG101 (ATG101 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AV13_pCAG.Cas9.gRNA.NT

    Plasmid

    #199260

    Purpose

    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG1.1-HA/Ppp1cc2

    Plasmid

    #199626

    Purpose

    Expression vector of mouse PPP1CC2. HA tag at N-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Ppp1cc mRNA (variant X1) (Ppp1cc Mouse)
    

    Tags

    HA-tag

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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