STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.
Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2. PubMed Article
Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, Owada H, Ohkawa Y, Pertsinidis A, Yamamoto T, Kimura H, Ochiai H
Nat Commun. 2022 Dec 20;13(1):7672. doi: 10.1038/s41467-022-35286-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174870 | eSpCas9-EF-5Flnc | CRISPR vector for generating Flnc STREAMING-tag KI cell |
| 174871 | eSpCas9-EF-5Nanog_2 | CRISPR vector for generating Nanog STREAMING-tag KI cell |
| 174872 | eSpCas9-EF-5Pou5f1 | CRISPR vector for generating Pou5f1 STREAMING-tag KI cell |
| 174873 | eSpCas9-EF-5Usp5 | CRISPR vector for generating Usp5 STREAMING-tag KI cell |
| 174874 | eSpCas9-EF-5Wnk1 | CRISPR vector for generating Wnk1 STREAMING-tag KI cell |
| 174876 | pLR5-CAG-42B3mutAC2(Ser2ph)-SNAP | PiggyBac transposon vector for expression of RNAPII Ser2ph mintbody |
| 174877 | pLR5-CAG-44B12mut23(Ser5ph)-SNAP | PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody |
| 174878 | pLR5-CAG-hMCP-mScarlet-I-NLS | PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS |
| 174879 | pLR5-CAG-NLS-SNAP | PiggyBac transposon vector for expression of NLS-SNAP |
| 174880 | pLR5-CAG-TetR_W43F-3xmNG | PiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreen |
| 174881 | pTV-Flnc-5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Flnc STREAMING-tag KI cell |
| 174882 | pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Nanog STREAMING-tag KI cell |
| 174883 | pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Pou5f1 STREAMING-tag KI cell |
| 174884 | pTV-Sox2_5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Sox2 STREAMING-tag KI cell |
| 174885 | pTV-Usp5-5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Usp5 STREAMING-tag KI cell |
| 174886 | pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR | Targeting vector for generating Wnk1 STREAMING-tag KI cell |
| 174887 | pBSKΔB-24xopto-TetO-no01 | Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag |
| 177265 | pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4 | STREAMING-tag cassette |
| 177267 | eSpCas9-EF | CRISPR vector |
| 177268 | pBSKΔB | Backbone vector for STREAMING-tag knockin vector |
| 186778 | PB533-44B12m23-sfGFP | RNAP2 Ser5ph-mintbody expression vector for mammalian cells |