We narrowed to 1,249 results for: pgem

1. pISH-mV2R55

   Plasmid

   #15866

   Depositor

   Peter Mombaerts

   Article

   Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

   Insert

   mV2R55
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2008

   Availability

   Academic Institutions and Nonprofits only

2. CRISPR-Cas9 Gateway System for Physcomitrella patens

   Plasmid Kit

   #1000000151

   Purpose

   CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.

   Depositor

   Magdalena Bezanilla

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pISH-Cux1

   Plasmid

   #74341

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   cut-like homeobox 1 (Cux1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HBV 1.3-mer P-null replicon

   Plasmid

   #65462

   Purpose

   containing 1.3 units of the P-null HBV genome (subtype ayw)

   Depositor

   Wang-Shick Ryu

   Article

   Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

   Insert

   1.3 units of the P-null HBV genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEn-Chimera

   Plasmid

   #61432

   Purpose

   Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLI-TGD(1463000)

   Plasmid

   #85791

   Purpose

   P. falciparum integration plasmid to disrupt genes using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PF3D7146300 targeting region, GFP-2TA-Neo-R
   

   Use

   P. falciparum genomic integration (gene disruptio…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   none

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-Cas9-gate

   Plasmid

   #113742

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hox D4a

    Plasmid

    #36469

    Depositor

    Robert Ho

    Article

    Prince et al Development. 1998 Feb . 125(3):407-20.

    Insert

    Hox D4a (hoxd4a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Bacterial

    Mutation

    Q100H, A101T, G125C and R189W

    Promoter

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pGolf-ISH

    Plasmid

    #15853

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type (Gnal Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    F199S

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pSLI-2xFKBP-GFP(1463000)

    Plasmid

    #85789

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    

    Use

    P. falciparum integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLI-sandwich(0807600)

    Plasmid

    #85790

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
    

    Use

    P. falciparum genomic integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pISH-Adnp

    Plasmid

    #74340

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Adnp. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    activity-dependent neuroprotective protein (Adnp Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEn-C1.1

    Plasmid

    #61479

    Purpose

    Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

    Depositor

    Holger Puchta

    Article

    Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLI-N-GFP-2xFKBP-loxP(K13)

    Plasmid

    #85792

    Purpose

    P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    Kelch13
    

    Use

    Cre/Lox; P. falciparum genomic integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZeo-Cas9-gate

    Plasmid

    #113744

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pISH-En2

    Plasmid

    #84310

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    En2 (En2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCngA2-ISH

    Plasmid

    #15855

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pISH-Emx2

    Plasmid

    #74346

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    empty spiracles homeobox 2 (Emx2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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