We narrowed to 118,634 results for: MPI

1. PB CAG-sfGFP-GSAx9-iCre-ERT2

   Plasmid

   #137857

   Purpose

   CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP-GSAx9-iCre-ERT2
   

   Use

   Cre/Lox; Piggybac

   Expression

   Mammalian

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. rox Neo* rox

   Plasmid

   #134322

   Purpose

   rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

   Depositor

   Mario Capecchi

   Insert

   Neo*
   

   Use

   Mouse Targeting; Dre/rox

   Expression

   Bacterial and Mammalian

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-HisFlag-rUSP19ER-CRmut

   Plasmid

   #155253

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) CR mutant (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Arg

   Promoter

   CMV

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HisFlag-rUSP19ER deltaN3

   Plasmid

   #155243

   Purpose

   for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HisFlag-rUSP19ER deltaN2

   Plasmid

   #155242

   Purpose

   for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-HisFlag-rUSP19ER deltaN1

   Plasmid

   #155241

   Purpose

   for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-536) (ER isoform) (Usp19 Rat)
   

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9id_TRP

   Plasmid

   #141254

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD289

   Plasmid

   #137018

   Purpose

   GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   GFP-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

   Plasmid

   #91803

   Purpose

   Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used

   Depositor

   Jeremy Wilusz

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. UCT1m

    Plasmid

    #121041

    Purpose

    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    UCT-part guide RNA (Stanley Qi sequence)
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. C114m

    Plasmid

    #121013

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    dCas9 with no bbsI or BsaI sites
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX335 Mouse 5' Srcap gRNA B

    Plasmid

    #127905

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX335 Mouse 5' Srcap gRNA A

    Plasmid

    #127904

    Purpose

    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

    Depositor

    Mark Groudine

    Insert

    Srcap gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 Mouse 3' Ubf gRNA

    Plasmid

    #127902

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Mouse 3' Ubf
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700-TTN-Puro

    Plasmid

    #128323

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against human TTN for activation
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZDonor-U6-myogenin stop codon gRNA

    Plasmid

    #69549

    Purpose

    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Insert

    Myogenin C terminus-targeting guide RNA
    

    Use

    CRISPR

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-Lin28a-as

    Plasmid

    #120528

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fly 2.0 pIDTBlue

    Plasmid

    #117418

    Purpose

    Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

    Depositor

    Dirk Dittmer

    Insert

    FLY 2.0
    

    Tags

    NA

    Expression

    Bacterial

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-EGFP-Lin28IR

    Plasmid

    #120517

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6Lin28a-as

    Plasmid

    #120536

    Purpose

    ssRNA expression for dsRNA production

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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