We narrowed to 32,570 results for: Eng

1. pGEMT-3P2A

   Plasmid

   #111772

   Purpose

   Quadcistronic construct: Co-express four genes by 3 P2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBFRE-luc

   Plasmid

   #26897

   Depositor

   Nicholas Gaiano

   Article

   Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

   Insert

   CBFRE
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pYPQ132-tRNA

   Plasmid

   #179211

   Purpose

   Golden Gate entry vector; 2nd gRNA with tRNA

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   no promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG03

   Plasmid

   #154138

   Purpose

   This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

   Depositor

   Kang Zhou

   Article

   Panda et al Biochemical Engineering Journal

   Insert

   fucO, aldA
   

   Expression

   Bacterial

   Promoter

   P_gyrA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-myc-Wnk1

   Plasmid

   #38779

   Depositor

   Melanie Cobb

   Article

   Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

   Insert

   Wnk1 (Wnk1 Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCM271rfp

   Plasmid

   #59774

   Purpose

   pCMrfp with TrfA mutation (271R to C)

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   RFP
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Tags

   his tag and sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-PCP-3XNeon-nls

   Plasmid

   #75386

   Purpose

   PCP-3XNeon-nls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   3XmNeonGreen

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLH-sgRNA1-2XboxB

   Plasmid

   #75391

   Purpose

   sgRNA1-2XboxB

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XboxB
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-BRIPO

    Plasmid

    #215542

    Purpose

    BRIPO (Bioluminescent Red Indicator for Potassium)

    Depositor

    Huiwang Ai

    Article

    Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

    Insert

    BRIPO
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Expression

    Bacterial

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIN-Ri

    Plasmid

    #197784

    Purpose

    Level 2 destination vector: pMIN-Ri backbone

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_CnCas12f1

    Plasmid

    #204996

    Purpose

    CnCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

    Insert

    CnCas12f1
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-MSCV-4xS1m-GFP-T2A-PU

    Plasmid

    #138581

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    S1m tag

    Promoter

    MSCV

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCWori-A13AMO-aaCPRct

    Plasmid

    #20117

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    Amorphadiene oxidase and p450 reductase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCM4.4

    Plasmid

    #217648

    Purpose

    an ermE*p-cas9-driven expression, as an alternative plasmid

    Depositor

    Huimin Zhao

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    Codon Optimized SpCas9
    

    Use

    CRISPR

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid

    #37184

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. His_CL7_2NLS_iCas12a-4NLS

    Plasmid

    #222971

    Purpose

    Bacterial expression of NLS-rich iCas12a construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His_CL7_2NLS_iCas12a-4NLS
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1090

    Plasmid

    #111427

    Purpose

    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA13-GG

    Plasmid

    #208002

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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