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pJP_Bla01

Plasmid

#230982

Purpose

Modified version of the pBLADE_ONLY_C (#168050). It expresses VVD-AraC, but the f1 ori was deleted and the chloramphenicol resistance gene was replaced by gentamicin resistance.

Depositor

Yolanda Schaerli

Article

Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

Insert

Gentamicin resistance gene

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p44PPV-eGFP

Plasmid

#241907

Purpose

Part 2 of a two-plasmid PCA biosensor composed of the PcaV repressible PPV promoter upstream from a reporter gene (eGFP) on a second plasmid

Depositor

Neil Dixon

Article

F M Machado et al J Biol Eng. 2019 Nov 27;13:91. doi: 10.1

Insert

His6-eGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_WT

Plasmid

#224142

Purpose

IPTG-inducible expression of C. elegans protein IDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1 (F59B8.2 Nematode)

Tags

intein-CBD

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_G98N

Plasmid

#224143

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(G98N) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(G98N)

Tags

intein-CBD

Expression

Bacterial

Mutation

G98N

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_R133H

Plasmid

#224144

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(R133H) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(R133H)

Tags

intein-CBD

Expression

Bacterial

Mutation

R133H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_T11_EGFP-Responder

Plasmid

#230051

Purpose

It presents the T11 inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

T11_EGFP

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_mCherry-Responder

Plasmid

#230052

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

mCherry

Expression

Mammalian

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_TRE3VG_EGFP-Responder

Plasmid

#230050

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of EGFP through the OPTi-OX platform

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

TRE3VG_EGFP

Expression

Mammalian

Promoter

GAAGACAATAGCAGGCATG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-NiV-F-WT-Foldon

Plasmid

#200391

Purpose

Mammalian cell expression of Nipah virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

NiV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pαH-LayV-F-WT-Foldon

Plasmid

#200392

Purpose

Mammalian cell expression of Langya virus fusion protein

Depositor

Priyamvada Acharya

Article

May et al J Virol. 2023 Jun 29;97(6):e0043323. doi

Insert

LayV-F Ectodomain

Tags

Foldon, HRV3C cleavage site, 8X His tag, 2X Strep…

Expression

Mammalian

Mutation

Ectodomain (AA 1-483)

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJV170

Plasmid

#180077

Purpose

Backbone to clone MTase genes for expression in TXTL

Depositor

Chase Beisel

Article

Vento et al Mol Cell. 2024 Jul 25;84(14):2785-2796.e

Type

Empty backbone

Expression

Bacterial

Promoter

P70a

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEP17

Plasmid

#229234

Purpose

PutP (Sodium/proline symporter) under tet operator, TetR

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Insert

putP (Sodium/proline symporter) (putP E. coli)

Expression

Bacterial

Promoter

tet operator

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHK401

Plasmid

#235482

Purpose

Repression of gene VIII by Plux/tet hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK201

Plasmid

#235481

Purpose

Repression of gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK328

Plasmid

#235479

Purpose

Inducible gene VIII by Pbad/lac hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK318

Plasmid

#235478

Purpose

Inducible gene VIII by Plux/tet hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK009

Plasmid

#235459

Purpose

NOR gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

NOR gate

Use

Synthetic Biology

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK506.4

Plasmid

#235455

Purpose

BUF/YES gate receiver with bs for sgRNA2

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

YES/BUF gate

Use

Synthetic Biology

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHK506.5

Plasmid

#235456

Purpose

BUF/YES gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

YES/BUF gate

Use

Synthetic Biology

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHK507

Plasmid

#235457

Purpose

OR/NAND gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

OR gate

Use

Synthetic Biology

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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