We narrowed to 59,457 results for: cloning

1. pENTR223-BZLF1(Zta)

   Plasmid

   #195787

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BZLF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BZLF1 (BZLF1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB515

   Plasmid

   #74857

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPS

   Plasmid

   #85801

   Purpose

   Backbone for pooled cloning of Perturb-seq libraries

   Depositor

   Aviv Regev

   Article

   Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWB418

   Plasmid

   #74812

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWB442

   Plasmid

   #74828

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB535

   Plasmid

   #74873

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGWB407

   Plasmid

   #74801

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

   Plasmid

   #193752

   Purpose

   human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-sgRNA-CBh-eGFP

    Plasmid

    #206885

    Purpose

    cloning backbone for sgRNA using OptScf2

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Type

    Empty backbone

    Promoter

    U6

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB444

    Plasmid

    #74830

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB553

    Plasmid

    #74883

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGWB544

    Plasmid

    #74878

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDarmo.CMVT_v1

    Plasmid

    #133072

    Purpose

    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-Flag-TwinStrep and TwinStrep-Flag-3C

    Expression

    Mammalian

    Promoter

    CMVT (Enhanced CMV)

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDA_722

    Plasmid

    #216077

    Purpose

    CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Destination

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VRK1_HUMAN_D0

    Plasmid

    #79684

    Purpose

    This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    VRK1 (VRK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. SlutrCas9

    Plasmid

    #163795

    Purpose

    Expresses SlutrCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-EBNA1

    Plasmid

    #195827

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA1 (EBNA-1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB453

    Plasmid

    #74835

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge